{{'Search' | translate}}
 

Low gelling temperature agarose

琼脂糖,低胶凝温度

Company: Sigma-Aldrich
Catalog#: A9414
Bio-protocol()
Company-protocol()
Other protocol()

A Robust Mammary Organoid System to Model Lactation and Involution-like Processes
Author:
Date:
2021-04-20
[Abstract]  

The mammary gland is a highly dynamic tissue that changes throughout reproductive life, including growth during puberty and repetitive cycles of pregnancy and involution. Mammary gland tumors represent the most common cancer diagnosed in women worldwide. Studying the regulatory mechanisms of mammary gland development is essential for understanding how dysregulation can lead to breast cancer initiation and progression. Three-dimensional (3D) mammary organoids offer many exciting possibilities for the study of tissue development and breast cancer. In the present protocol derived from Sumbal et al., we describe a straightforward 3D organoid system for the study of lactation and involution ex vivo. We use primary and passaged mouse mammary organoids stimulated with fibroblast growth factor 2

...
[摘要]  
[摘要]乳腺是一种高度动态的组织,在整个生殖生活中都会发生变化,包括青春期的生长以及怀孕和进化的重复周期。乳腺肿瘤诊断代表在世界女性最常见的癌症宽。研究的监管机制乳腺的发育是至关重要的理解荷兰国际集团d如何YS调节可导致乳腺癌的发生和发展。三维(3D)乳腺组织体提供了许多令人激动的可能性的研究的组织发育和乳腺癌。在第E存在衍生自协议Sumbal等人,我们描述一个简单的3D类器官系统的研究的泌乳和复古体外。我们使用成纤维细胞生长因子2 (FGF2)和催乳素刺激的原代和传代小鼠乳腺类器官来模拟小鼠乳腺泌乳和内卷过程的三个周期。这种3D模型类器官代表一个有价值的工具来研究后期产后乳腺的发育和乳腺癌,尤其是产后-相关性乳腺癌。


图形摘要:


乳腺类器官的分离和培养程序

[背景技术]的Th e是乳腺的主要功能是提供营养吨经由牛奶产量Ò新生儿。牛逼乳腺他的发展是主要发生在出生后,由几个因素,包括激素和生长因子调控的一个高度动态的过程(Brisken和拉贾拉姆,2006;斯特恩利希特,2006年)。在青春期,激素和生长因子调节基本的胚胎导管树的导管形态发生(Brisken and ...

Staining and Quantitative Analysis of Myelinating Oligodendrocytes in the Mouse Grey Matter
Author:
Date:
2020-10-20
[Abstract]  Oligodendrocytes generate distinct patterns of myelination throughout the CNS. Variations in myelination along axons may enable neurons to fine-tune conduction velocities and alter signal synchronisation. Here we outline a staining protocol permitting the assessment of the number and length of myelin sheaths formed by oligodendrocyte in the mouse grey matter. This protocol enables the investigation of myelination without the need for reporter mice or technically challenging protocols, aiding the investigation of factors influencing myelin production in the brain. [摘要]  [摘要] 少突胶质细胞在中枢神经系统产生不同的髓鞘形成模式。轴突髓鞘的变化可能使神经元能够微调传导速度和改变信号同步。在这里,我们概述了一个染色方案,允许评估由少突胶质细胞在小鼠灰质中形成的髓鞘的数量和长度。这一方案使研究髓鞘无需报告小鼠或技术上具有挑战性的协议,有助于研究影响大脑髓鞘生成的因素。

[背景] 少突胶质细胞在中枢神经系统(CNS)轴突周围产生绝缘的髓鞘。髓鞘通过鞘间小的无髓鞘间隙处电压依赖性钠通道的浓度加速轴突传导速度——Ranvier节点(Huxley和Stampfli,1949;Rushton,1951;Waxman,1997)。尽管少突胶质细胞遍布中枢神经系统,但并非所有轴突都有髓鞘,这表明髓鞘的形成和大小可能精确地调节动作电位速度和神经元同步性(Pajevic等人,2014)。因此,了解是什么影响少突胶质细胞产生髓鞘的数量(即一个细胞形成的鞘的数量和大小)对于理解髓鞘如何改变神经元功能很重要。
许多技术已经被发展用来分析体内少突胶质细胞的复杂形态。最初,Pio del Rio Hortega的研究使用碳酸银染色法,根据形成的髓鞘的数量和长度来识别和区分少突胶质细胞(Perez ...

Zebrafish Embryo Xenograft and Metastasis Assay
Author:
Date:
2018-09-20
[Abstract]  Xenograft models, and in particular the mouse xenograft model, where human cancer cells are transplanted into immunocompromised mice, have been used extensively in cancer studies. Although these models have contributed enormously to our understanding of cancer biology, the zebrafish xenograft model offers several advantages over the mouse model. Zebrafish embryos can be easily cultured in large quantities, are small and easy to handle, making it possible to use a high number of embryos for each experimental condition. Young embryos lack an efficient immune system. Therefore the injected cancer cells are not rejected, and the formation of primary tumors and micrometastases is rapid. Transparency of the embryos enables imaging of primary tumors and metastases in an intact and living embryo. ... [摘要]  异种移植模型,特别是小鼠异种移植模型,其中人癌细胞被移植到免疫受损小鼠中,已广泛用于癌症研究。尽管这些模型对我们对癌症生物学的理解做出了巨大贡献,但斑马鱼异种移植模型与鼠标模型相比具有多种优势。斑马鱼胚胎可以很容易地大量培养,体积小且易于处理,因此可以在每种实验条件下使用大量胚胎。年轻的胚胎缺乏有效的免疫系统。因此,注射的癌细胞不被排斥,并且原发性肿瘤和微转移的形成是快速的。胚胎的透明度使得能够在完整和活的胚胎中对原发性肿瘤和转移瘤进行成像。在这里,我们描述了一种方法,其中表达GFP的肿瘤细胞被注射到斑马鱼胚胎的心包空间。在注射后4天,对胚胎成像并分析原发性肿瘤和远端微转移的形成。

【背景】 斑马鱼( Danio rerio )是一种小型淡水鱼,不仅在发育生物学中,而且在生物医学中越来越受欢迎。快速发展的领域之一是使用斑马鱼作为癌症生物学研究的模型。斑马鱼高效可靠地交配,可以产生大量小,透明,外部发育的后代,可以在例如,多孔板中轻松培养(White et al。 >,2013)。有趣的是,人类肿瘤细胞可以植入斑马鱼胚胎中,许多异种移植物能够通过在胚胎中生长,侵入和转移来忠实地重演它们的恶性行为(Lee et al。,2005; Nicoli et al。,2007; Chapman et ...

Comments