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Taq DNA Polymerase

Taq DNA聚合酶

Company: ROBOKLON
Catalog#: E2500
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Detection of DNA Methylation Changes Surrounding Transposable Elements
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Date:
2013-06-05
[Abstract]  Transposable elements (TEs) are a major component of all genomes, thus the epigenetic mechanisms controlling their activity is an important field of study. Cytosine methylation is one of the factors regulating the transcription and transposition of TEs, alongside Histone modifications and small RNAs. Adapter PCR-based methods [such as Amplified Fragment Length Polymorphism (AFLP)] have been successfully used as high-throughput methods to genotype un-sequenced genomes. Here we use methylation-sensitive restriction enzymes, in combination with PCR on adaptor-ligated restriction fragments, to evaluate epigenetic changes in TEs between genomic DNA samples. [摘要]  转座元件(TE)是所有基因组的主要组成部分,因此控制其活性的表观遗传机制是重要的研究领域。 胞嘧啶甲基化是调节TE的转录和转座,与组蛋白修饰和小RNA一起的因素之一。 基于适配子PCR的方法[例如扩增片段长度多态性(AFLP)]已经成功地用作高通量方法来对未测序的基因组进行基因分型。 在这里我们使用甲基化敏感的限制酶,结合适配器连接的限制性片段上的PCR,以评估基因组DNA样品之间的TE的表观遗传变化。

High Resolution Detection of Genetic Changes Associated with Transposons
Author:
Date:
2013-06-05
[Abstract]  Transposable elements (TEs) are repetitive sequences, capable of inducing genetic mutations through their transpositional activity, or by non-homologous or illegitimate recombination. Because of their similarity and often high copy numbers, examining the effects of mutations caused by TEs in different samples (tissues, individuals, species, etc.) can be difficult. Thus, high throughput methods have been developed for genotyping TEs in un-sequenced genomes. A common method is termed Transposon Display (or transposon SSAP), which utilizes restriction enzymes and PCR amplification to produce chimeric DNA molecules that include genomic and TE DNA. The advent of second generation sequencing technologies, such as 454-pyrosequencing, have dramatically improved the resolution of this assay, ... [摘要]  转座元件(TE)是重复序列,能够通过其转座活性或通过非同源或非同源重组诱导遗传突变。 由于它们的相似性和通常高的拷贝数,检查由不同样品(组织,个体,物种等)中TE引起的突变的影响可能是困难的。 因此,已经开发了用于在未测序的基因组中对TE进行基因分型的高通量方法。 常见的方法称为转座子展示(或转座子SSAP),其利用限制酶和PCR扩增来产生包括基因组和TE DNA的嵌合DNA分子。 第二代测序技术(例如454焦磷酸测序)的出现已显着改善了该测定的分辨率,允许同时测序所有PCR产物,代表特定基因组中的所有扩增的TE位点。

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