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PageRulerTM Unstained Protein Ladder

PageRuler TM未染色蛋白梯度

Company: Thermo Fisher Scientific
Catalog#: 26614
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2D Diagonal Redox SDS-PAGE of Proteins
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2013-06-05
[Abstract]  2D diagonal redox SDS-PAGE of proteins is used to detect intramolecular or intermolecular disulfide bridges using Chlamydomonas in this example (Stroeher and Dietz, 2008; Schwarz et al., 2012). Both dimensions consist of a conventional SDS-PAGE, except that the sample buffer for the first dimension lacks a reducing agent. Intermolecular disulfide bridges increase the apparent molecular weight of a protein in the first dimension, whereas intramolecular bridges decrease the apparent weight of the protein. [摘要]  在该实施例中,使用蛋白质的2D对角线氧化还原SDS-PAGE用于检测分子内或分子间二硫键(Stroeher和Dietz,2008; Schwarz等人,2012) 。 两个维度由常规SDS-PAGE组成,除了第一维的样品缓冲液不含还原剂。 分子间二硫桥在第一维中增加蛋白质的表观分子量,而分子内桥降低蛋白质的表观重量。

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