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Author:
Date:
2012-03-05
[Abstract] The scratch wound healing assay has been widely adapted and modified to study the effects of a variety of experimental conditions, for instance, gene knockdown or chemical exposure, on mammalian cell migration and proliferation. In a typical scratch wound healing assay, a “wound gap” in a cell monolayer is created by scratching, and the “healing” of this gap by cell migration and growth towards the center of the gap is monitored and often quantitated. Factors that alter the motility and/or growth of the cells can lead to increased or decreased rate of “healing” of the gap (Lampugnani, 1999). This assay is simple, inexpensive, and experimental conditions can be easily adjusted for different purposes. The assay can also be used for a high-throughput screen platform if an automated system ...
[摘要] 划伤伤口愈合测定法已经广泛适应和修改以研究多种实验条件例如基因敲低或化学暴露对哺乳动物细胞迁移和增殖的影响。 在典型的伤口创伤愈合测定中，通过刮擦产生细胞单层中的"伤口间隙"，并且监测并常常定量通过细胞迁移和朝向间隙中心的生长的该间隙的"愈合"。 改变细胞运动性和/或生长的因素可导致间隙"愈合"的增加或减少的速率（Lampugnani，1999）。 该测定简单，便宜，并且可以容易地针对不同目的调整实验条件。 如果使用自动化系统，该测定也可以用于高通量筛选平台（Yarrow和Perlman，2004）。
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