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Dimethyl pimelimidate dihydrochloride

庚二亚胺二盐酸盐

Company: Sigma-Aldrich
Catalog#: D8388
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Immunoprecipitation for Cell Culture
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2012-02-05
[Abstract]  Immunoprecipitation (IP) is a method to pull down a protein out of solution using an antibody that specifically binds to that particular protein. Immunoprecipitation is a powerful technique to isolate and concentrate a particular protein from a sample containing many thousands of different proteins, to test protein-protein interactions, and to pull multiple members of a complex out of solution by latching onto one member with an antibody. This protocol describes a general immunoprecipitation strategy using cell cultures as starting material. [摘要]  免疫沉淀(IP)是使用特异性结合该特定蛋白的抗体将蛋白从溶液中取出的方法。 免疫沉淀是从含有数千种不同蛋白质的样品中分离和集中特定蛋白质,测试蛋白质 - 蛋白质相互作用,以及通过用抗体锁定到一个成员上而将复合物的多个成员从溶液中抽出的强大技术。 该协议描述了使用细胞培养物作为起始材料的一般免疫沉淀策略。

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