| Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-cardiolipin Antibodies
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Author:
Date:
2012-06-20
[Abstract] The circulating anticardiolipin antibody is a hallmark of antiphospholipid syndrome. It also appears in a number of autoimmune mouse models and is indicative of the break of tolerance against self antigens. This protocol describes a reliable method to determine the relative serum titer of anticardiolipin in autoimmune mouse models.
[摘要] 循环抗心磷脂抗体是抗磷脂综合征的标志。 它也出现在许多自身免疫小鼠模型中，并且指示对自身抗原的耐受性的破坏。 该协议描述了一种可靠的方法来确定抗心磷脂在自身免疫小鼠模型中的相对血清滴度。
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| Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-histone Antibodies
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Author:
Date:
2012-06-20
[Abstract] Autoimmune disease is characterized with the break of tolerance against self antigens. Anti-histone antibodies can be found in the majority of patients with system lupus erythematosus (SLE) and a number of lupus-prone mouse strains. This protocol describes a reliable method to determine the relative serum titers of anti-histone antibodies in these mice.
[摘要] 自身免疫性疾病的特征在于对自身抗原的耐受性的中断。 抗组蛋白抗体可以在系统性红斑狼疮（SLE）和许多易患狼疮的小鼠品系的大多数患者中发现。 该方案描述了确定这些小鼠中抗组蛋白抗体的相对血清滴度的可靠方法。
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| Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-dsDNA Antibodies
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Author:
Date:
2011-02-20
[Abstract] ELISA is an easy and relatively sensitive way to measure protein concentration. This protocol describes how to measure serum levels of murine anti-dsDNA antibodies. Circulating anti-dsDNA antibody is a hallmark of SLE both in human patients and in many SLE mouse models. This protocol was developed or modified in Dr. Anne Davidson’s lab at Feinstein Institute for Medical Research.
[摘要] ELISA是测量蛋白质浓度的简单且相对灵敏的方法。 该协议描述如何测量鼠抗dsDNA抗体的血清水平。 循环抗dsDNA抗体是人类患者和许多SLE小鼠模型中SLE的标志。 这个协议是在费恩斯坦医学研究所的Anne Davidson博士的实验室中开发或修改的。
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