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Sodium phosphate dibasic


Company: Sigma-Aldrich
Catalog#: S3397
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Open-book Preparations from Chick Embryos and DiI Labeling of Commissural Axons
[Abstract]  Successful neural circuit formation relies on the accurate navigation of axons towards their targets during development. Axons are guided by a combination of short-range and long-range, attractive and repulsive cues. The commissural axons of the developing spinal cord have provided an informative in vivo model for the identification of multiple axon guidance molecules and mechanisms. These axons extend ventrally from the dorsal spinal cord and cross the midline at the floor plate, before making a sharp rostral turn towards the head. This simple trajectory has facilitated the identification of many axon guidance molecules, because perturbation of the stereotypical guidance decisions as a result of genetic manipulations can be easily identified. The open-book assay is a method to ... [摘要]  成功的神经电路形成依赖于在发展期间轴突朝向其靶标的精确导航。轴突由短程和长程,吸引和排斥线索的组合引导。发育中的脊髓的连合轴突提供了用于多轴突导向分子和机制的鉴定的信息的体内模型。这些轴突从背侧脊髓向腹侧延伸并在底板处穿过中线,然后朝向头部进行尖锐的嘴唇转向。这个简单的轨迹有助于许多轴突指导分子的识别,因为可以容易地识别作为遗传操作的结果的定型指导决定的干扰。开放书测定是评估脊柱连合轴突的轨迹的方法。脊髓被解剖出来​​,在屋顶板上打开并固定平。亲脂性荧光染料DiI的点状注射用于在显微镜和分析之前追踪连合轴突轨迹。

Detection of Hydrogen Peroxide by DAB Staining in Arabidopsis Leaves
[Abstract]  In this protocol, the in situ detection of hydrogen peroxide (one of several reactive oxygen species) is described in mature Arabidopsis rosette leaves by staining with 3,3'-diaminobenzidine (DAB) using an adaptation of previous methods (Thordal-Christensen et al., 1997; Bindschedler et al., 2006; Daudi et al., 2012). DAB is oxidized by hydrogen peroxide in the presence of some haem-containing proteins, such as peroxidases, to generate a dark brown precipitate. This precipitate is exploited as a stain to detect the presence and distribution of hydrogen peroxide in plant cells. The protocol can be modified slightly to detect hydrogen peroxide in different types of plant tissue. [摘要]  在该方案中,在成熟的拟南芥花瓣叶中通过用3,3'-二氨基联苯胺(1mM)染色来描述过氧化氢(几种活性氧种类之一)的原位检测。 DAB),使用先前方法的适应(Thordal-Christensen等人,1997; Bindschedler等人,2006; Daudi等人, ,2012)。 DAB在一些含血红蛋白(例如过氧化物酶)的存在下被过氧化氢氧化,以产生深棕色沉淀。 利用该沉淀作为污渍来检测植物细胞中过氧化氢的存在和分布。 该方案可以稍微修改以检测不同类型的植物组织中的过氧化氢。