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Drosophila S2 Cells in Schneider's Medium

Drosophila S2 Cells in Schneider''s Medium

Company: Thermo Fisher Scientific
Catalog#: R69007
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Transfection of S2 Cell with DNA Using CellFectin Reagent
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2012-03-05
[Abstract]  This method provides a step-by-step guide to transfecting Drosophila S2 cells with the pRmHA-3 (or similar) vector with insert of choice (in this case SDF-1β-FLAG) and generating a stable cell line. This cell line is then capable of producing the protein of interest under inducible conditions by addition of copper sulfate, which can then be purified and used as desired. This protocol provides an example to finding out when your peak protein production occurs and a method for determining optimal selection conditions. [摘要]  该方法提供了用具有选择插入片段(在这种情况下是SDF-1β-FLAG)的pRmHA-3(或相似)载体转染果蝇S2细胞并产生稳定细胞系的逐步指导。 然后,该细胞系能够在诱导条件下通过加入硫酸铜产生目的蛋白,然后可以根据需要纯化和使用。 这个协议提供了一个例子,找出什么时候你的蛋白质峰的产生和一个确定最佳选择条件的方法。

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