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MaxQTM 2000 and 3000 Benchtop Orbital Shakers

MaxQ TM 2000和3000 Benchtop Orbital Shakers

Company: Thermo Fisher Scientific
Catalog#: SHKA2000
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Induction, Isolation and Counting of Akinetes in Aphanizomenon ovalisporum
[Abstract]  Akinetes are spore-like resting (dormant) cells formed by strains of filamentous cyanobacteria for surviving long periods of unfavorable conditions. During deprivation for potassium, vegetative photosynthetic cells along the filaments of the cyanobacterium Aphanizomenon ovalisporum (A. ovalisporum) (strain ILC-164) differentiate into akinetes. Akinetes are larger than vegetative cell, have a thick wall, accumulate storage compounds (cyanophycine, glycogen, lipids) and excess of DNA (Sukenik et al., 2015; Sukenik et al., 2007; Maldener et al., 2014). Differences in structure and composition between akinetes and vegetative cells allow separation and isolation of akinetes. Akinetes isolated by the described protocol can be utilized for protein ... [摘要]  Akinetes是由丝状蓝细菌菌株形成的孢子样休眠(休眠)细胞,用于在长期不利条件下存活。 在剥夺钾期间,沿着蓝细菌Aphanizomenon ovalisporum( ovlisporum )(菌株ILC-164)的细丝的营养光合细胞分化成动力基因。 Akinetes大于营养细胞,具有厚壁,积累储存化合物(氰基霉素,糖原,脂质)和过量的DNA(Sukenik等人,2015; Sukenik等人, em>,2007; Maldener等人,,2014)。 动力学和营养细胞之间的结构和组成的差异允许动力蛋白的分离和分离。 通过所述方案分离的Akinet可以用于蛋白质分析,代谢活性测量,荧光原位杂交(FISH)研究等。