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SYTOX® Green Nucleic Acid Stain - 5 mM Solution in DMSO

SYTOX® Green Nucleic Acid Stain - 5 mM Solution in DMSO

Company: Thermo Fisher Scientific
Catalog#: S7020
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In vitro Detection of Neutrophil Traps and Post-attack Cell Wall Changes in Candida Hyphae
Author:
Date:
2017-04-05
[Abstract]  In this protocol we describe how to visualize neutrophil extracellular traps (NETs) and fungal cell wall changes in the context of the coculture of mouse neutrophils with fungal hyphae of Candida albicans. These protocols are easily adjusted to test a wide array of hypotheses related to the impact of immune cells on fungi and the cell wall, making them promising tools for exploring host-pathogen interactions during fungal infection. [摘要]  在本协议中,我们描述了如何在小鼠嗜中性粒细胞与真菌白色念珠菌的真菌菌丝共培养的背景下观察嗜中性粒细胞胞外捕获物(NETs)和真菌细胞壁变化。 这些方案很容易调整,以测试与免疫细胞对真菌和细胞壁的影响有关的各种假设,使其成为探索真菌感染期间宿主病原体相互作用的有前景的工具。

C。白色念珠菌是一种多态性机会性酵母,嗜中性粒细胞是免疫细胞,对于这种和其他真菌病原体的防御至关重要(Brown等,2012; Lionakis和Netea,2013)。 NETs是可以针对病原体部署的潜在防御机制,并且已经表明它们优先针对诸如C的微生物细胞部署。白念珠菌菌丝太大而不能吞噬(Urban et al。,2006; Bruns等人,2010; Branzk等人,,2014; Rohm等人,2014)。 NET已经显示含有许多组分,包括髓过氧化物酶,胞外DNA和瓜氨酸化的组蛋白(Amulic等人,2012; ...

Generation of Tumour-stroma Minispheroids for Drug Efficacy Testing
Author:
Date:
2017-01-05
[Abstract]  The three-dimensional organisation of cells in a tissue and their interaction with adjacent cells and extracellular matrix is a key determinant of cellular responses, including how tumour cells respond to stress conditions or therapeutic drugs (Elliott and Yuan, 2011). In vivo, tumour cells are embedded in a stroma formed primarily by fibroblasts that produce an extracellular matrix and enwoven with blood vessels. The 3D mixed cell type spheroid model described here incorporates these key features of the tissue microenvironment that in vivo tumours exist in; namely the three-dimensional organisation, the most abundant stromal cell types (fibroblasts and endothelial cells), and extracellular matrix. This method combined with confocal microscopy can be a powerful tool to ... [摘要]  组织中细胞的三维组织及其与相邻细胞和细胞外基质的相互作用是细胞反应的关键决定因素,包括肿瘤细胞如何对应激条件或治疗药物的反应(Elliott和Yuan,2011)。在体内,肿瘤细胞被包埋在主要由成纤维细胞形成的基质中,所述成纤维细胞产生细胞外基质并用血管编织。这里描述的3D混合细胞类型球体模型包括了体内存在肿瘤的组织微环境的这些关键特征;即三维组织,最丰富的基质细胞类型(成纤维细胞和内皮细胞)和细胞外基质。该方法结合共聚焦显微镜可以成为不同肿瘤类型的药物敏感性,血管生成和细胞迁移/侵袭测定的有力工具。

背景 传统的单层细胞培养(二维)强化人造环境,其与体内存在的组织细胞大不相同。最重要的区别之一是在单层培养物中,细胞是极化的,即,面向培养物的细胞表面和暴露于培养基的上细胞表面完全不同,经常反对的信号(Fitzgerald等人,2015)。为了解决细胞极化的问题,肿瘤球状体培养越来越多地用于癌症研究。肿瘤球体可以通过减少其通常发生在单层培养物中的生长培养基的扩散和稀释,通过细胞因子和趋化因子复制存在于组织中的三维细胞 - 细胞相互作用和一定程度的旁分泌信号传导(Lawlor等,2002; ...

Estimation of the Chromosomal Copy Number in Synechococcus elongatus PCC 7942
Author:
Date:
2016-07-05
[Abstract]  Cyanobacteria are prokaryotic organisms that perform oxygenic photosynthesis. Freshwater cyanobacteria, such as Synechococcus elongatus PCC 7942 and Synechocystis sp. PCC 6803, are model organisms for the study of photosynthesis, gene regulation, and biotechnological applications because they are easy to manipulate genetically. However, while studying these cyanobacteria, care has to be taken with respect to genetic heterogeneity in the establishment of gene disruptants, because these cyanobacteria contain multiple chromosomal copies per cell. Here, we describe a method for the estimation of chromosomal copy number in Synechococcus 7942. Using this method, we have recently observed that the chromosomal copy number of Synechococcus 7942 ... [摘要]  蓝细菌是进行含氧光合作用的原核生物。 淡水蓝细菌,例如细长聚球藻PCC 7942和聚集细胞 PCC 6803是用于研究光合作用,基因调节和生物技术应用的模式生物,因为它们易于基因操作。 然而,当研究这些蓝细菌时,必须注意遗传异质性在基因破坏物的建立中,因为这些蓝细菌每个细胞含有多个染色体拷贝。 在这里,我们描述了用于估计在聚球藻7942中的染色体拷贝数的方法。使用这种方法,我们最近观察到,聚球藻7942的染色体拷贝数显着地改变 其生长阶段。 这种技术可用于研究多倍体不仅在蓝细菌,而且在其他多倍体生物。

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