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Hydroxylamine hydrochloride


Company: Sigma-Aldrich
Catalog#: 159417
Other protocol()

Rust Removal Experiments
[Abstract]  Iron oxidation, known as rust formation, causes enormous loss in term of property damages and associated economic risks. Depending on the degree of formation, rust consists of several layers of iron in different oxidation states. The brownish top layer is mostly iron (III) oxide-hydroxide [FeO(OH), Fe(OH)3] while the deepest black layers possess iron oxide (Fe2O3.nH2O). The flaky nature of surface rust meditates diffusion of oxygen and water to inner material sections which can lead to total disintegration of iron mass. As a result, it is desirable to remove rust and protect fresh surface from oxidizers. The common rust removal reagents are mainly based on complex formation of ferric ion with organic and inorganic acids such as citric ... [摘要]  铁氧化,被称为生锈,导致财产损失和相关的经济风险的巨大损失。根据形成的程度,锈由几层不同氧化态的铁组成。褐色顶层主要是氧化铁(III) - 氧化物[FeO(OH),Fe(OH)3],而最深的黑色层具有氧化铁(Fe 2 O 3) O 3 nH O)。表面锈蚀的片状性质沉积了氧气和水扩散到内部材料部分,这可能导致铁质的完全分解。因此,希望除去锈和保护新鲜表面免受氧化剂的影响。常见的除锈剂主要基于三价铁离子与有机和无机酸如柠檬酸,草酸和磷酸的络合物形成。除锈能力通常是定性观察,其使得治疗选择之间的直接比较繁琐(如果不是不切实际的话)。在我们最近的工作(Ahmadi等人,2015)中,我们开发了一种比色测定法,用于测量除铁处理介质中的铁浓度,使用细菌产生的铁载体(yersiniabactin)与商业生锈去除试剂。在该方法中,铁浓度与在不同去除剂的存在下溶解的锈的质量相关。该测定基于1,10-菲咯啉测定(Skoog和West,1979)的修饰,以使得能够使用96孔板格式进行更高通量筛选和评估的检测。

Assay of Ornithine Decarboxylase and Spermidine/Spermine N1-acetyltransferase Activities
[Abstract]  The polyamines, spermidine (Spd) and spermine, and their diamine precursor putrescine, are important regulators of various cellular functions, such as proliferation and differentiation. Polyamine homeostasis is tightly regulated on the level of uptake, excretion, biosynthesis, interconversion and terminal catabolism. The rate-controlling enzymes of polyamine biosynthesis and interconversion are ornithine decarboxylase (ODC) and spermidine/spermine N1-acetyltransferase (SSAT), respectively. Here, we describe a protocol to assay ODC (Jänne and Williams-Ashman, 1971) and SSAT (Libby, 1978) activities from cell or tissue samples. [摘要]  多胺,亚精胺(精)和精胺及其二胺前体腐胺是各种细胞功能(如增殖和分化)的重要调节剂。 多胺稳态在摄取,排泄,生物合成,相互转化和末端分解代谢的水平上受到严格调节。 多胺生物合成和相互转化的速率控制酶分别是鸟氨酸脱羧酶(ODC)和亚精胺/精胺N 1 - 乙酰基转移酶(SSAT)。 在这里,我们描述了测定细胞或组织样品的ODC(Jänne和Williams-Ashman,1971)和SSAT(Libby,1978)活性的方案。