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4-Nitrophenyl α-L-arabinofuranoside


Company: Sigma-Aldrich
Catalog#: N3641
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Assay of Arabinofuranosidase Activity in Maize Roots
[Abstract]  Root is a perfect model for studying the mechanisms of plant cell growth. Along the root length, several zones where cells are at different stages of development can be visualized (Figure 1). The dissection of the root on these zones allows the investigation of biochemical and genetic aspects of different growth steps. Maize primary root is much more massive than the root of other Monocots and thus more convenient for such type of research. Plant cell wall, mainly consisting of polysaccharides, plays an important role in plant life. Therefore, measurement of plant carbohydrate content and glycoside-modifying enzyme activity in plant cells has become an important aspect in plant physiology. One of the well-documented changes of hemicelluloses molecules during elongation growth of monocots ... [摘要]  根是研究植物细胞生长机制的完美模型。沿着根长度,可以可视化细胞处于不同发育阶段的几个区(图1)。在这些区域上的根的解剖允许调查不同生长步骤的生化和遗传方面。玉米主根比其他单根的根更大,因此这种类型的研究更方便。植物细胞壁,主要由多糖组成,在植物生活中发挥重要作用。因此,植物碳水化合物含量和糖苷修饰酶活性在植物细胞中的测量已经成为植物生理学中的重要方面。在单子叶植物细胞的延长生长期间半纤维素分子的充分证明的变化之一是葡糖醛酸阿拉伯木聚糖的阿拉伯糖取代的减少。这可能是由于该多糖的合成的变化或阿拉伯呋喃糖苷酶的作用引起的。在这里,我们描述的分光光度测量阿拉伯呋喃糖苷酶活性在玉米根中的水解发色底物(4-硝基苯基α-L-阿拉伯呋喃糖苷)的速率的协议。

图1. 四日龄黑暗生长的玉米幼苗面板)。根据Kozlova等人(2012)(右图),初级玉米根的不同区和细胞发育的相应阶段。