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Company: Duchefa Biochemie
Catalog#: D1004
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Infection Assay of Cyst Nematodes on Arabidopsis Roots
[Abstract]  Plant parasitic nematodes are devastating pests on many crops. Juveniles (J2) of cyst nematodes invade the roots to induce a syncytium. This feeding site is their only source of nutrients. Male nematodes leave the roots after the fourth molt to mate with females. The females stay attached to their syncytia throughout their life and produce hundreds of eggs, which are contained in their bodies. When the females die their bodies form the cysts, which protect the eggs. Cysts can survive for many years in the soil until favorable conditions induce hatching of the juveniles.

The beet cyst nematode Heterodera schachtii is a pathogen of sugar beet (Beta vulgaris) but can also complete its life cycle on Arabidopsis roots growing on agar plates under ...
[摘要]  植物寄生线虫是许多作物上的毁灭性害虫。 孢囊线虫的幼虫(J2)侵入根以诱导合胞体。 这个饲养场是他们唯一的营养来源。 雄性线虫在第四蜕皮后离开根以与雌性交配。 雌性在其整个生命期间保持附着于其合胞体,并产生数百个鸡蛋,其包含在它们的身体中。 当女性死于他们的身体形成囊肿,保护卵。 囊肿可以在土壤中存活多年,直到有利条件诱导幼鱼孵化。
甜菜孢囊线虫 Heterodera schachtii 是甜菜的病原体( vulgaris ),但也可以在拟南芥 根在无菌条件下在琼脂平板上生长。 我们在这里介绍的H的股票文化的协议。 schachtii)和在琼脂平板上的感染测定。

Agrobacterium-mediated Transformation of Strawberry
[Abstract]  Traditional breeding for improvement of strawberry (Fragaria x ananassa) is difficult because strawberry is an octoploid, hybrid species. Genetic modification of strawberry would though be a promising alternative for obtaining the desired improvements in existing elite strawberry cultivars (Schaart et al., 2011). The availability of suitable genes for trait improvements in strawberry has however been a rate-limiting step until recently, but with the completion of the sequencing of the genome of woodland strawberry (F. vesca) (Shualev et al., 2011), we now have access to a treasure chest with valuable candidate genes. For strawberry, methods for genetic transformation have originally been described by Nehra et al. (1990) and James et al. ... [摘要]  由于草莓是八倍体,杂种物种,用于改善草莓( Fragaria x ananassa )的传统育种是困难的。草莓的遗传修饰虽然是获得现有的优良草莓栽培品种所期望的改进的有希望的替代方法(Schaart等人,2011)。然而,随着草地草莓基因组测序的完成(Shualev ),用于草莓中性状改善的合适基因的可用性是速率限制步骤 et al。,2011),我们现在可以获得宝贵的候选基因宝库。对于草莓,遗传转化的方法最初由Nehra等人(1990)和James等人(1990)描述,并且转化的成功被证明是高度的品种依赖。草莓转化的最新进展由Husaini等人审查(2011)。在我们的实验室中,草莓的转化是基于由Passey等人(2003)描述的苗再生方法和使用超强毒力土壤杆菌菌株AGL0(Lazo et al。,1991)。我们主要利用草莓转化作为候选基因功能分析的工具。为此,栽培品种Calypso是非常合适的基因型,因为其高转化效率(高达100%)和永不结实的特性,一旦植物开始开花,其提供草莓果实的连续供应。