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120 C18 chromatographic column

120 C18色谱柱

Company: Thermo Fisher Scientific
Catalog#: 059130
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Camalexin Quantification in Arabidopsis thaliana Leaves Infected with Botrytis cinerea
[Abstract]  Phytoalexins are heterogeneous low molecular mass secondary metabolites with antimicrobial activity produced at the infection site in response to pathogen invasion and represent an important part of the plant defense repertoire. Camalexin (3-Thiazol-2′-yl-indole) is a known phytoalexin first detected and isolated in Camelina sativa, from which it takes its name, infected with Alternaria brassicae (Browne et al., 1991). Production of camalexin is also induced in Arabidopsis thaliana leaves by a range of biotrophic and necrotrophic plant pathogens (bacteria, oomycetes, fungi and viruses) (Ahuja et al., 2012) as well as by abiotic stresses, such as UV and chemicals (e.g. acifluorfen, paraquat, chlorsulfuron and α-amino butyric acid) (Zhao et ... [摘要]  植物素是具有抗菌活性的异质低分子量次级代谢物,其在感染部位响应于病原体侵入产生并且代表植物防御所有组成部分的重要部分。 Camalexin(3-噻唑-2'-基 - 吲哚)是已知的植物抗毒素,首先在Camelina sativa中检测和分离,从其命名,感染了链格孢菌 (Browne等人,1991)。 camalexin的产生也通过一系列生物营养和坏死的植物病原体(细菌,卵菌,真菌和病毒)在拟南芥叶中诱导(Ahuja等人,2012)以及非生物胁迫,例如紫外线和化学品(例如氟锁草醚,百草枯,氯磺隆和α-氨基丁酸)(Zhao等人,1998; Tierens等人, et al。,,2002)。 Camalexin源自色氨酸,CYP79B2和CYP71B15(PAD3)是催化其生物合成途径中的重要步骤的P450酶(Glawischnig,2007)。
camalexin含量的检测和定量需要了解它是如何在各种应激条件下产生的。在这里我们介绍camalexin提取从拟南芥叶片感染的坏死性真菌灰霉病,和进一步通过液相色谱 - 质谱(LC-MS)的camalexin水平的测定方法。该方法足够敏感,足以将痕量的camalexin降至低皮克(10pg/mg FW)范围。