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Fixation/Permeabilization Solution Kit with BD GolgiStopTM

固定/渗透溶液套装与BD GolgiStop TM

Company: BD
Catalog#: 554715
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Osteoblast Sorting and Intracellular Staining of CXCL12
Author:
Date:
2018-05-20
[Abstract]  Osteoblasts are bone marrow endosteum-lining niche cells playing important roles in the regulation of hematopoietic stem cells by secreting factors and cell adhesion molecules. Characterization of primary osteoblasts has been achieved through culture of outgrowth of collagenase treated bone. Immunophenotyping and flow-based analysis of long bone osteoblasts offer a simplified and rapid approach to characterize osteoblasts. We describe a modified procedure of isolating mouse bone marrow osteoblastic cells based on cell surface immunophenotyping. The chemokine CXCL12 (also known as stromal-derived factor, SDF-1) together with its receptor CXCR4 are expressed by osteoblasts and bone marrow stroma cells. The CXCL12-CXCR4 axis is important for hematopoietic stem cell retention to their niches ... [摘要]  成骨细胞是通过分泌因子和细胞粘附分子在调节造血干细胞中发挥重要作用的骨髓内皮细胞生态位细胞。 通过培养胶原酶处理的骨骼的生长已经实现了原代成骨细胞的表征。 长骨成骨细胞的免疫分型和基于流动的分析为表征成骨细胞提供了简化和快速的方法。 我们描述了基于细胞表面免疫分型的分离小鼠骨髓成骨细胞的修改过程。 趋化因子CXCL12(也称为基质衍生因子SDF-1)与其受体CXCR4一起由成骨细胞和骨髓基质细胞表达。 CXCL12-CXCR4轴对于造血干细胞滞留于其生态位(Sugiyama等,2006)和支持白血病启动细胞活性(Pitt等,2015年)。 这里我们描述CXCL12细胞内染色的过程。

【背景】骨髓龛是一种高度组织化的微环境,基质细胞参与与调节HSC静止,分化和动员的造血干细胞(HSC)的直接细胞 - 细胞相互作用(Anthony and Link,2014; Mendelson and Frenette,2014; Morrison和斯卡登,2014年)。 HSC细胞生态位中的多种细胞类型可能以不同的但可能重叠的方式贡献于小生境功能支持。这些细胞包括但不限于成骨细胞,破骨细胞,富含CXCL12的网状(CAR)细胞,Nestin +基质细胞,瘦素受体+(LepR + ...

Lymphocyte Isolation, Th17 Cell Differentiation, Activation, and Staining
Author:
Date:
2016-12-05
[Abstract]  In vitro Th17 (α, β T helper cell which produce IL-17A, IL-17F and IL-22) differentiation has been routinely used for functional T cells studies. Here we describe a method for Th17 cell differentiation. [摘要]   Th17(产生IL-17A,IL-17F和IL-22的α,βT辅助细胞)分化已经常规用于功能性T细胞研究。 这里我们描述Th17细胞分化的方法。
关键词: Th17,IL-17,FACS

[背景] T细胞 是介导宿主防御细菌,病毒和真菌以及共生的关键(Kumar等人,2016)。 T细胞可以基于它们产生特异性细胞因子的能力进一步细分为T辅助(Th1),Th2和Th17子集。 幼稚T细胞可以响应于特异性细胞因子刺激在体外培养中分化成特异性T细胞亚群。 体外产生的Th1,Th2和Th17细胞已经帮助我们理解它们的分化和它们的效应子功能的分子机制。 在这里,我们描述了Th17细胞生成的基本协议。

Intracellular Cytokine (INF-gamma) Staining Assay
Author:
Date:
2012-04-05
[Abstract]  An intracellular cytokine (INF-gamma) staining assay is used to analyze the function of lymphocytes at the single cell level. By combining surface staining and intracellular cytokine staining, this assay can reveal the percentage of cytokine-releasing cells in a particular population, which cannot be obtained from an ELISpot assay. [摘要]  细胞内细胞因子(INF-γ)染色测定用于分析淋巴细胞在单细胞水平的功能。 通过组合表面染色和细胞内细胞因子染色,该测定可以揭示特定群体中细胞因子释放细胞的百分比,其不能从ELISpot测定获得。

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