{{'Search' | translate}}

Adhesive Carbon Tape 8mm x 20m

胶带碳带8mm x 20m

Company: Agar Scientific
Catalog#: AGG3939
Other protocol()

In situ Digestion of Wheat Cell-wall Polysaccharides
[Abstract]  Cell walls of the wheat endosperm are mostly composed of arabinoxylans (AX) and mixed (1→3), (1→4)-β-glucans (BG) (Saulnier et al., 2012). Here, we present an optimized protocol to degrade enzymatically these cell-wall polysaccharides into oligosaccharides, directly from wheat grain cross sections. The main difficulty is to provide a sufficient amount of humidity for the enzyme to be active, while the amount of liquid at the surface of the tissue should stay low to prevent any delocalization of the released products. With this protocol, enzymatic degradation was shown to be efficient and delocalization of released oligosaccharides was estimated below 50 µm (Veličković et al., 2014).

Although it can be employed for other purposes, this in situ ...
[摘要]  小麦胚乳的细胞壁主要由阿拉伯木聚糖(AX)和混合的(1→3),(1→4)-β-葡聚糖(BG)组成(Saulnier等人,2012)。在这里,我们提出一个优化的协议,以酶法将这些细胞壁多糖降解为低聚糖,直接从小麦籽粒横截面。主要的困难是提供足够量的湿度以使酶活性,而组织表面处的液体量应保持低以防止释放的产物的任何离域。使用该方案,酶降解显示是有效的,并且释放的寡糖的离域化估计低于50μm(Veličkovic等人,2014)。虽然它可以用于其他目的,但是这种原位酶消化主要用于通过基质辅助激光解吸/电离(MALDI)获得小麦胚乳的横截面的分子图像)质谱(Veličkovic等人,2014)。细胞壁多糖在结构上是异质的,表现出高质量并且缠结到复杂网络中。因此,它们不适合通过质谱法直接分析,并且它们需要作为第一步骤降解为较小的化合物。在该方案中,还描述了对应于MALDI基质的沉积的另外的步骤。