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RPMI 1640 with L-glutamine

RPMI 1640与L-谷氨酰胺

Company: Lonza
Catalog#: 12-702F
Other protocol()

Isolation and in vitro Activation of Mouse Peyer’s Patch Cells from Small Intestine Tissue
[Abstract]  The lumen of gastrointestinal tract is exposed to several potentially pathogenic microorganisms, thus it is extremely relevant to understand how immunosurveilance can be established. Peyer’s Patches (PPs) are oval or round lymphoid nodules that protrude from the outer wall of the ileum portion of small intestine. PPs contain a high percentage of B and T lymphocytes, macrophages and dendritic cells. Here we summarize a protocol for isolation and culture of mouse PP cells, which can be used to get a better insight into immunopathologies of microbes and to evaluate immune responses elicited by mucosal vaccines. [摘要]  胃肠道的腔暴露于几种潜在的致病微生物,因此,了解如何建立免疫耐受是非常重要的。 派尔氏贴剂(PP)是椭圆形或圆形淋巴结,其从小肠的回肠部分的外壁突出。 PP含有高百分比的B和T淋巴细胞,巨噬细胞和树突细胞。 在这里我们总结一个协议的小鼠PP细胞的分离和培养,可用于更好地了解微生物的免疫病理学和评估由粘膜疫苗引发的免疫反应。