{{'Search' | translate}}
 

NuncTM Dishes with HydroCellTM Surface

Nunc TM菜肴与HydroCell TM表面

Company: Thermo Fisher Scientific
Catalog#: 174912
Bio-protocol()
Company-protocol()
Other protocol()

Isolation and Culture of Neurospheres for the Study of Pathogenesis of Prion Disease
Author:
Date:
2014-03-20
[Abstract]  Neurosphere contains neural stem cells that are capable of self-renewal and multilineage differentiation including neurons, astrocytes, and oligodendrocytes (Gage, 2000). Cell culture model using differentiated neurosphere cultures are suggested to be a valuable tool for studying the pathogenesis of prion disease at the cellular level (Iwamaru et al., 2013). This protocol describes the procedure for a culture of whole brain-derived neurospheres from newborn mouse brains. Neurosphere formation steadily occurs within a week from the cultures of neonatal whole brains and these cells have stem cell properties. [摘要]  神经球包含能够自我更新和多向分化的神经干细胞,包括神经元,星形胶质细胞和少突胶质细胞(Gage,2000)。 使用分化的神经球培养物的细胞培养模型被认为是用于研究朊病毒疾病在细胞水平的发病机制的有价值的工具(Iwamaru等人,2013)。 该协议描述了来自新生小鼠脑的全脑衍生的神经球的培养的程序。 神经球形成稳定地发生在新生儿全脑的培养物的一周内,并且这些细胞具有干细胞特性。

Neural Stem Cell Differentiation and Prion Infection
Author:
Date:
2014-03-20
[Abstract]  Prion diseases are transmissible, fatal, neurodegenerative diseases in human and animals. The molecular basis of neurodegeneration in prion diseases is largely unclear. Developing a cellular model capable of monitoring prion-induced cytotoxicity would be a promising approach for better understanding the prion pathogenesis. One candidate cellular assay is a model based on neurospheres, which contains neural stem cells (NSCs). Both undifferentiated and differentiated NSCs have been demonstrated to be permissive to prion infection, and prion-induced cytopathic changes in differentiated neruosphere cultures were reported (Iwamaru et al., 2013). This protocol describes the procedure to induce differentiation of NSCs from transgenic mice overexpressing prion protein (tga20 mice) into ... [摘要]  朊病毒疾病是人类和动物中可传播的,致命的,神经变性疾病。 朊病毒疾病中神经变性的分子基础很大程度上不清楚。 开发能够监测朊病毒诱导的细胞毒性的细胞模型将是更好地理解朊病毒发病机制的有前途的方法。 一种候选细胞测定是基于神经球的模型,其含有神经干细胞(NSC)。 未分化和分化的NSCs都被证明是容许朊病毒感染,并且报道了分化的神经球培养物中的朊病毒诱导的细胞病变变化(Iwamaru等人,2013)。 该协议描述了诱导NSCs从过度表达朊病毒蛋白(tga20小鼠)的转基因小鼠分化成易感染朊病毒感染的培养物的过程。

Comments