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PerCP Streptavidin


Company: BD
Catalog#: 554064
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LT-HSC Methylcellulose Assay
[Abstract]  Hematopoietic differentiation is a highly complex process originating from an extraordinary population of cells called long-term repopulating hematopoietic stem cells (LT-HSCs). The unique feature of all stem cells, including HSCs, is their exceptional ability to divide asymmetrically giving rise to two different kinds of offspring. One daughter cell becomes an LT-HSC itself (self-renews) to maintain the LT-HSC pool, whereas the second daughter cell pursues a differentiation fate to ultimately give rise to terminally differentiated mature blood cells (Orkin and Zon, 2008). Quantification of phenotypic LT-HSCs can be performed by multi-color flow cytometry and the gold standard for assessment of LT-HSC self-renewal and function is competitive bone marrow transplantation (Miller et al. ... [摘要]  造血分化是一种非常复杂的过程,源自称为长期重建造血干细胞(LT-HSCs)的非凡细胞群。所有干细胞(包括HSC)的独特之处在于其不对称的划分能够产生两种不同种类的后代。一个子细胞成为LT-HSC本身(自我更新)以维持LT-HSC库,而第二个子细胞追求分化命运,最终产生终末分化的成熟血细胞(Orkin和Zon,2008)。表型LT-HSC的定量可以通过多色流式细胞术进行,用于评估LT-HSC自我更新和功能的黄金标准是竞争性骨髓移植(Miller等,2008)。尽管这些方法对于确定LT-HSC丰度和功能是不可替代的,但它们具有其缺点和局限性。例如,竞争性骨髓移植通常被监测为12-16周内外周血献血者贡献的函数。虽然减少的外周血供体贡献本身表示干/祖细胞隔室中的损伤,但是它不能明确区分降低的LT-HSC自我更新,受损的LT-HSC分化或受损的祖细胞分化。这里我们描述一种LT-HSCs甲基纤维素集落形成测定法,作为直接评估LT-HSC分化能力的快速互补体外方法。如Kerenyi等人所述(2013),该技术是区分LT-HSC或祖细胞分化缺陷的有力工具。

Isolation of Dendritic Cells and Macrophages from the Murine Kidneys of Lupus by Cell Sorter
[Abstract]  Methods for the isolation and characterization of mononuclear phagocytes from the kidneys of mice with SLE are essential to understand the patho-physiology of the disease. Activation of these cells is associated with the onset of clinical disease in mice and infiltration with these cells is associated with poor prognosis in humans.An analysis of the function of these cells should lead to a better understanding of the inflammatory processes that lead to renal impairment in SLE and other renal inflammatory diseases.
[摘要]  从SLE小鼠的肾中分离和表征单核吞噬细胞的方法对于了解疾病的病理生理学是必不可少的。 这些细胞的活化与小鼠中的临床疾病的发作相关,并且这些细胞的浸润与人类的不良预后相关。这些细胞的功能的分析应导致更好地理解导致肾损伤的炎症过程 在SLE和其他肾脏炎性疾病中